HCR RNA-FISH: Protocol Overview
The same 2-stage protocol is used independent of the number of target RNAs.
All major
automation platforms
Simple Robust Protocols
HCR™ northern blot protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target RNAs.
​
Automatic Background Suppression
HCR™ northern blot reagents provide automatic background suppression throughout the protocol, ensuring that even if probes or hairpins bind non-specifically in the sample they will not generate amplified background, dramatically enhancing performance and ease-of-use.
​
Straightforward Multiplexing
HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all target RNAs simultaneously.
​
Custom Probe Design
Free custom probe design is available for any target mRNA in any organism across the tree of life.
​
Normal Sleep Schedule
We favor protocols with an overnight probe incubation step to enable researchers to maintain a normal sleep schedule; durations can be customized as desired.
Quantitative Band Intensities
HCR™ northern blots enable relative and absolute quantitation of RNA target abundance.
HCR™ Northern Blot Protocol
Multiplexed quantitative RNA blotting
HCR™ northern blot probe sets, amplifiers, and buffers enable multiplexed quantification of RNA target size and abundance with automatic background suppression throughout the protocol (how HCR™ northern blots work).
HCR™ Northern Blots: Protocol Overview
Detection Stage
Amplification Stage
The same 2-stage protocol is used independent of the number of target RNAs.
