HCR™ IF Technology
Multiplexed, quantitative, high-resolution protein imaging
HCR™ probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunofluorescence (IF), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunostaining.
HCR™ IF: How It Works
Protein targets are detected with unlabeled primary antibody probes and HCR™ probe comprising an initiator-labeled secondary antibody.
An HCR™ amplifier comprises metastable fluorescent hairpins h1 and h2. Initiator i1 triggers self-assembly of tethered fluorescent amplification polymers.
Simple Robust Protocols
HCR™ IF protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.
HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.
Plug-and-Play with Your 1º Antibodies of Choice
For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes.
HCR™ Protein-IF + HCR™ RNA-FISH
Combine HCR™ IF probes with HCR™ RNA-FISH probes to enable HCR™ protein-IF + HCR™ RNA-FISH for multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR™ signal amplification for all protein and RNA targets simultaneously.
Subcellular Quantitative Protein Imaging
Subcellular protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.