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HCR™ IF Technology

Multiplexed, quantitative, high-resolution protein imaging

HCR™ probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunofluorescence (IF), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunostaining.

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HCR™ IF: How It Works

Detection Stage

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Amplification Stage

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Detection Stage

Protein targets are detected with unlabeled primary antibody probes and HCR™ probe comprising an initiator-labeled secondary antibody. 

Amplification Stage

An HCR™ amplifier comprises metastable fluorescent hairpins h1 and h2. Initiator i1 triggers self-assembly of tethered fluorescent amplification polymers. 

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Experience Multiplexed, Quantitative, High-Resolution
Immunofluorescence

Amplified. Enzyme-Free. FFPE. Simple.

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Simple Robust Protocols

HCR™ IF protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.

Straightforward Multiplexing

HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.

Plug-and-Play with Your 1º Antibodies of Choice

For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes. 

HCR™ Protein-IF + HCR™ RNA-FISH

Combine HCR™ IF probes with HCR™ RNA-FISH probes to enable HCR™ protein-IF + HCR™ RNA-FISH for multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR™ signal amplification for all protein and RNA targets simultaneously. 

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Subcellular Quantitative Protein Imaging

Subcellular protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

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The same 2-stage enzyme-free protocol is used independent of the number of target proteins

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Protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples

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Bidirectional quantitative protein discovery in an anatomical context

HCR™ IF

HCR™ initiator-labeled 2º antibody probes, HCR™ amplifiers, HCR™ buffers
Plug-and-play with your own 1º antibodies of choice (without modification) 
Multiplexed, quantitative, high-resolution
FFPE tissue sections
Whole-mount embryos

All major 

automation platforms 

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