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HCR™ RNA-FISH

A new era for in situ hybridization: 

multiplexed, quantitative, high-resolution, sensitive, versatile, robust

HCR™ RNA-FISH
Multiplexed, quantitative, high-resolution RNA imaging 

Multiplexed Experiment

  • Order one HCR™ RNA-FISH bundle per target RNA

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Example 2-Plex Experiment

  • ​HCR™ RNA-FISH bundle for target mRNA1

    • HCR™ probe set: target mRNA1 for use with amplifier B1

    • HCR™ amplifier: B1-647

    • HCR™ RNA-FISH buffers: probe hybridization buffer, probe wash buffer, amplification buffer (for use with all bundles) 

  • HCR™ RNA-FISH bundle for target mRNA2

    • HCR™ probe set: target mRNA2 for use with amplifier B2​

    • ​HCR™ amplifier: B2-488​

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The same probe sets and amplifiers enable multiplexed quantitative RNA flow cytometry and northern blotting

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HCR™ IF

Experience multiplexed, quantitative, high-resolution immunfluorescence: amplified, enzyme-free, FFPE, simple

HCR™ IF
Multiplexed, quantitative, high-resolution protein imaging 

Multiplexed Experiment

  • Order one HCR™ IF bundle per target protein

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Example 2-Plex Experiment

  • ​HCR™ IF bundle for target Protein1

    • 1º Ab: Rabbit Anti-Protein1 (your own 1º antibody) 

    • HCR™ 2º Ab probe: Donkey Anti-Rabbit for use with amplifier B1

    • HCR™ amplifier: B1-647

    • HCR™ IF buffers: antibody buffer, amplification buffer (for use with all bundles) 

  • HCR™ IF bundle for target Protein2

    • 1º Ab: Mouse Anti-Protein2 (your own 1º antibody)

    • HCR™ 2º Ab probe: Donkey Anti-Mouse for use with amplifier B2

    • HCR™ amplifier: B2-488

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HCR™ IF + HCR™ RNA-FISH

Quantitative 1-step enzyme-free HCR™ signal amplification for all protein and RNA targets simultaneously

HCR™ IF + HCR™ RNA-FISH
Simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging 

Multiplexed Experiment

  • Order one HCR™ IF bundle per target protein

  • Order one HCR™ RNA-FISH bundle per target RNA 

​

Example 2-Plex Experiment

  • HCR™ RNA-FISH bundle for target mRNA1

    • HCR™ probe set: target mRNA1 for use with amplifier B1

    • HCR™ amplifier: B1-647

    • HCR™ RNA-FISH buffers: probe hybridization buffer, probe wash buffer, amplification buffer 

  • HCR™ IF bundle for target Protein1

    • 1º Ab: Mouse Anti-Protein1 (your own 1º antibody)

    • HCR™ 2º Ab probe: Donkey Anti-Mouse for use with amplifier B2

    • HCR™ amplifier: B2-488

    • ​HCR™ IF buffers: antibody buffer, amplification buffer 

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