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A new era for in situ hybridization: 

multiplexed, quantitative, high-resolution, sensitive, versatile, robust

HCR RNA-FISH
Multiplexed, quantitative, high-resolution RNA imaging 

Multiplexed Experiment

  • Order one HCR RNA-FISH bundle per target RNA

Example 2-Plex Experiment

  • ​HCR bundle for target mRNA1

    • HCR split-initiator probe set: target mRNA1 for use with amplifier B1

    • HCR amplifier: B1-647

    • HCR RNA-FISH buffers: probe hybridization buffer, probe wash buffer, amplification buffer (for use with all bundles) 

  • HCR bundle for target mRNA2

    • HCR split-initiator probe set: target mRNA2 for use with amplifier B2​

    • ​HCR amplifier: B2-488​

The same reagents enable multiplexed quantitative RNA flow cytometry and northern blotting

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Experience multiplexed, quantitative, high-resolution immunhistochemistry: amplified, enzyme-free, FFPE, simple

HCR IHC
Multiplexed, quantitative, high-resolution protein imaging 

Multiplexed Experiment

  • Order one HCR IHC bundle per target protein

Example 2-Plex Experiment

  • ​HCR bundle for target Protein1

    • 1º Ab: Rabbit Anti-Protein1 (your own 1º antibody) 

    • HCR 2º Ab probe: Donkey Anti-Rabbit for use with amplifier B1

    • HCR amplifier: B1-647

    • HCR IHC buffers: antibody buffer, amplification buffer (for use with all bundles) 

  • HCR bundle for target Protein2

    • 1º Ab: Mouse Anti-Protein2 (your own 1º antibody)

    • HCR 2º Ab probe: Donkey Anti-Mouse for use with amplifier B2

    • HCR amplifier: B2-488

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Quantitative 1-step enzyme-free HCR signal amplification for all protein and RNA targets simultaneously

HCR IHC + HCR RNA-FISH
Simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging 

Multiplexed Experiment

  • Order one HCR IHC bundle per target protein

  • Order one HCR RNA-FISH bundle per target RNA 

Example 2-Plex Experiment

  • HCR bundle for target mRNA1

    • HCR split-initiator probe set: target mRNA1 for use with amplifier B1

    • HCR amplifier: B1-647

    • HCR RNA-FISH buffers: probe hybridization buffer, probe wash buffer, amplification buffer 

  • HCR bundle for target Protein1

    • 1º Ab: Mouse Anti-Protein1 (your own 1º antibody)

    • HCR 2º Ab probe: Donkey Anti-Mouse for use with amplifier B2

    • HCR amplifier: B2-488

    • ​HCR IHC buffers: antibody buffer, amplification buffer