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HCR™ Gold RNA-FISH

A new era for in situ hybridization:

10-plex, quantitative, high-resolution, sensitive, versatile, robust, enzyme-free

HCR™ Gold RNA-FISH
The gold standard for RNA imaging
10-plex, quantitative, high-resolution, enzyme-free RNA fluorescence in situ hybridization (RNA-FISH) with automatic background suppression throughout the protocol for unmatched versatility and robustness

Multiplex Experiment

  • Order one HCR™ Gold RNA-FISH kit per target RNA (select a different HCR™ Gold Amplifier for each target RNA)

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Example 2-Plex Experiment

  • ​HCR™ Gold RNA-FISH kit for target RNA1

    • HCR™ HiFi Probe: for target RNA1 for use with amplifier X3

    • HCR™ HiFi Probe Hybridization Buffer (for use with all kits)

    • HCR™ HiFi Probe Wash Buffer (for use with all kits) ​

    • HCR™ Gold Amplifier: X3 with label 647

    • HCR™ Gold Amplifier Buffer (for use with all kits) 

    • HCR™ Gold Amplifier Wash Buffer (for use with all kits) 

  • HCR™ Gold RNA-FISH kit for target RNA2

    • HCR™ HiFi Probe: target RNA2 for use with amplifier​ X1

    • ​HCR™ Gold Amplifier: X1 with label 488​

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The same HCR™ HiFi Probes and HCR™ Gold Amplifiers enable multiplex quantitative HCR™ Gold RNA Flow Cytometry and HCR™ Gold Northern Blots

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HCR™ Gold IF

Game-changing multiplex immunofluorescence using same-species 1º antibodies with the HCR™ HiFi Encoder

HCR™ Gold IF
Game-changing multiplex protein imaging
Use the breakthrough HCR™ HiFi Encoder to robustly encode best-in-class quantitative signal amplification to your favorite 1º antibodies for same-species/isotype multiplex immunofluorescence (IF)

Multiplex Experiment

  • Use your own trusted 1º antibodies (same host species/isotype for each target protein if desired; e.g., 10-plex experiment using Rabbit IgG 1º antibodies for all 10 target proteins)

  • Order one HCR™ Gold IF kit per target protein (select a different HCR™ HiFi Encoder and HCR™ Gold Amplifier for each target protein) 

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Example 2-Plex Experiment

  • ​HCR™ Gold IF kit for target Protein1

    • 1º antibody: Rabbit IgG Anti-Protein1 (your own 1º antibody) 

    • HCR™ HiFi Encoder: Rabbit IgG for amplifier X3

    • HCR™ HiFi Antibody Buffer (for use with all kits)​

    • HCR™ HiFi Antibody Wash Buffer (for use with all kits)

    • HCR™ Gold Amplifier: X3 with label 647

    • HCR™ Gold Amplifier Buffer (for use with all kits)

    • HCR™ Gold Amplifier Wash Buffer (for use with all kits) 

  • HCR™ Gold IF kit for target Protein2

    • 1º antibody: Rabbit IgG Anti-Protein2 (your own 1º antibody)

    • HCR™ HiFi Encoder: Rabbit IgG for amplifier X1

    • HCR™ Gold Amplifier: X1 with label 488

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HCR™ Gold RNA-FISH/IF

Quantitative 1-step enzyme-free HCR™ Gold signal amplification for 10 RNA and/or protein targets simultaneously

HCR™ Gold RNA-FISH/IF
Unified multiplex, quantitative, high-resolution RNA and protein imaging 
Combine the game-changing performance and flexibility of HCR™ Gold RNA-FISH and HCR™ Gold IF with 1-step quantitative signal amplification for 10 RNA and/or protein targets simultaneously

Multiplex Experiment​

  • Order one HCR™ Gold RNA-FISH kit per target RNA

  • Order one HCR™ Gold IF kit per target protein (same host species/isotype for each target protein if desired)

  • Select a different HCR™ Gold Amplifier for each target RNA or protein

​

Example 2-Plex Experiment

  • HCR™ Gold RNA-FISH kit for target RNA1

    • HCR™ HiFi Probe: for target RNA1 for use with amplifier X3

    • HCR™ HiFi Probe Hybridization Buffer

    • HCR™ HiFi Probe Wash Buffer 

    • HCR™ Gold Amplifier: X3 with label 647

    • HCR™ Gold Amplifier Buffer (for use with all kits) 

    • HCR™ Gold Amplifier Wash Buffer (for use with all kits) 

  • HCR™ Gold IF kit for target Protein1

    • 1º antibody: Rabbit IgG Anti-Protein1 (your own 1º antibody)

    • HCR™ HiFi Encoder: Rabbit IgG for amplifier X1

    • HCR™ HiFi Antibody Buffer​

    • HCR™ HiFi Antibody Wash Buffer

    • HCR™ Gold Amplifier: X1 with label 488​

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HCR™ Pro RNA-ISH

Enzymatic amplification with chromogenic or fluorescent staining for your most demanding targets/samples

HCR™ Pro RNA-ISH
Extreme-sensitivity RNA imaging with chromogenic or fluorescent staining
Use enzymatic amplification for the most demanding targets/samples when signal-to-background is at an absolute premium (e.g., rapid scanning of a low-expression target in a highly-autofluorescent sample over a large field-of-view at low magnification)​

Example 1-Plex Experiment

  • HCR™ HiFi Probe: for target RNA1 for use with amplifier X2

  • HCR™ HiFi Probe Hybridization Buffer

  • HCR™ HiFi Probe Wash Buffer

  • HCR™ Pro Amplifier kit​: X2

    • Pretreat

    • HCR™ Pro Detect A​

    • HCR™ Pro Detect B​

    • HCR™ Pro Detect C​

    • HCR™ Pro Detect D​

    • HCR™ Pro Detect E​

    • HCR™ Pro Detect F HRP

    • Post-Process

  • HCR™ Pro Amplifier Wash Buffer

  • Matisse® chromogen kit

    • Matisse® Green or Matisse® Brown 

    • Matisse® Green Buffer or Matisse® Brown Buffer

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The HCR™ Pro RNA-ISH kit is used with an HRP chromogen for RNA-CISH (e.g., Matisse® Green or Matisse® Brown) or an HRP fluorogenic substrate for RNA-FISH (e.g., any of a variety of recommended third-party fluorescent tyramides).

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HCR™ Gold 2ºIF

Multiplex, quantitative, high-resolution IF using different-species 1º antibodies with HCR™ 2º Antibody Probes

HCR™ Gold 2ºIF
Multiplex, quantitative, high-resolution protein imaging 
Use your favorite 1º antibodies without modification (must use a different host species/isotype for each target protein) in combination with validated HCR™ 2º Antibody Probes

Multiplex Experiment

  • use your own trusted 1º antibodies (different host species/isotype for each target protein)

  • order one HCR™ Gold 2ºIF kit per target protein (select a different HCR™ 2º Antibody Probe and HCR™ Gold Amplifier for each target protein)

​

Example 2-Plex Experiment

  • ​HCR™ Gold 2ºIF kit for target Protein1

    • 1º antibody: Rabbit Anti-Protein1 (your own 1º antibody) 

    • HCR™ 2º Antibody Probe: Donkey Anti-Rabbit for use with amplifier X3

    • HCR™ Antibody Buffer (for use with all kits)​

    • HCR™ Gold Amplifier: X3 with label 647

    • HCR™ Gold Amplifier Buffer (for use with all kits)

    • HCR™ Gold Amplifier Wash Buffer (for use with all kits) 

  • HCR™ Gold 2ºIF kit for target Protein2

    • 1º antibody: Mouse Anti-Protein2 (your own 1º antibody)

    • HCR™ 2º Antibody Probe: Donkey Anti-Mouse for use with amplifier X1

    • HCR™ Gold Amplifier: X1 with label 488

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HCR™ Gold RNA-FISH/2ºIF

Combine HCR™ Gold RNA-FISH and HCR™ Gold 2º IF (using different-species 1º antibodies for each target protein)

HCR™ Gold RNA-FISH/2ºIF
Unified multiplex, quantitative, high-resolution RNA and protein imaging 
Combine HCR™ Gold RNA-FISH and HCR™ Gold 2ºIF (must use a different host species/isotype for each target protein) with 1-step quantitative signal amplification for 10 RNA and/or protein targets simultaneously

Multiplex Experiment​

  • Order one HCR™ Gold RNA-FISH kit per target RNA

  • Order one HCR™ Gold 2ºIF kit per target protein (different host species/isotype for each target protein)

  • Select a different HCR™ Gold Amplifier for each target RNA or protein

​

Example 2-Plex Experiment

  • HCR™ Gold RNA-FISH kit for target RNA1

    • HCR™ HiFi Probe: for target RNA1 for use with amplifier X3

    • HCR™ HiFi Probe Hybridization Buffer

    • HCR™ HiFi Probe Wash Buffer 

    • HCR™ Gold Amplifier: X3 with label 647

    • HCR™ Gold Amplifier Buffer (for use with all kits) 

    • HCR™ Gold Amplifier Wash Buffer (for use with all kits) 

  • HCR™ Gold 2ºIF kit for target Protein1

    • 1º antibody: Mouse Anti-Protein1 (your own 1º antibody)

    • HCR™ 2º Antibody Probe: Donkey Anti-Mouse for use with amplifier X1

    • HCR™ Antibody Buffer​

    • HCR™ Gold Amplifier: X1 with label 488​

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