A new era for in situ hybridization:
10-plex, quantitative, high-resolution, sensitive, versatile, robust, enzyme-free
HCR™ Gold RNA-FISH
Multiplex, quantitative, high-resolution RNA imaging
Multiplex Experiment
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Order one HCR™ Gold RNA-FISH kit per target RNA
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Example 2-Plex Experiment
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​HCR™ Gold RNA-FISH kit for target RNA1
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HCR™ HiFi Probe: for target RNA1 for use with amplifier X3
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HCR™ HiFi Probe Hybridization Buffer (for use with all kits)
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HCR™ HiFi Probe Wash Buffer (for use with all kits) ​
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HCR™ Gold Amplifier: X3 with label 647
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HCR™ Gold Amplifier Buffer (for use with all kits)
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HCR™ Gold Amplifier Wash Buffer (for use with all kits)
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HCR™ Gold RNA-FISH kit for target RNA2
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HCR™ HiFi Probe: target RNA2 for use with amplifier​ X1
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​HCR™ Gold Amplifier: X1 with label 488​
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The same HCR™ HiFi Probes and HCR™ Gold Amplifiers enable multiplex quantitative HCR™ Gold RNA Flow Cytometry and HCR™ Gold Northern Blots
Enzymatic amplification with chromogenic or fluorescent staining for your most demanding targets/samples
HCR™ Pro RNA-ISH
Extreme-sensitivity RNA imaging with chromogenic or fluorescent staining
Example 1-Plex Experiment
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HCR™ HiFi Probe: for target RNA1 for use with amplifier X2
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HCR™ HiFi Probe Hybridization Buffer
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HCR™ HiFi Probe Wash Buffer
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HCR™ Pro Amplifier kit​: X2
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Pretreat
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HCR™ Pro Detect A​
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HCR™ Pro Detect B​
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HCR™ Pro Detect C​
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HCR™ Pro Detect D​
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HCR™ Pro Detect E​
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HCR™ Pro Detect F HRP
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Post-Process
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HCR™ Pro Amplifier Wash Buffer
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Matisse® chromogen kit
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Matisse® Green or Matisse® Brown
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Matisse® Green Buffer or Matisse® Brown Buffer
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The HCR™ Pro RNA-ISH kit is used with an HRP chromogen for RNA-CISH (e.g., Matisse® Green or Matisse® Brown) or an HRP fluorogenic substrate for RNA-FISH (e.g., any of a variety of recommended third-party fluorescent tyramides).
Experience multiplex, quantitative, high-resolution immunfluorescence: amplified, enzyme-free, FFPE, simple
HCR™ Gold IF
Multiplex, quantitative, high-resolution protein imaging
Multiplex Experiment
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Order one HCR™ Gold IF kit per target protein
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Example 2-Plex Experiment
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​HCR™ Gold IF kit for target Protein1
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1º antibody: Rabbit Anti-Protein1 (your own 1º antibody)
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HCR™ 2º Antibody Probe: Donkey Anti-Rabbit for use with amplifier X3
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HCR™ Antibody Buffer (for use with all kits)​
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HCR™ Gold Amplifier: X3 with label 647
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HCR™ Gold Amplifier Buffer (for use with all kits)
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HCR™ Gold Amplifier Wash Buffer (for use with all kits)
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HCR™ Gold IF kit for target Protein2
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1º antibody: Mouse Anti-Protein2 (your own 1º antibody)
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HCR™ 2º Antibody Probe: Donkey Anti-Mouse for use with amplifier X1
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HCR™ Gold Amplifier: X1 with label 488
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Quantitative 1-step enzyme-free HCR™ Gold signal amplification for all RNA and protein targets simultaneously
HCR™ Gold RNA-FISH/IF
Simultaneous multiplex, quantitative, high-resolution RNA and protein imaging
Multiplex Experiment​
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Order one HCR™ Gold RNA-FISH kit per target RNA
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Order one HCR™ Gold IF kit per target protein
​
Example 2-Plex Experiment
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HCR™ Gold RNA-FISH kit for target RNA1
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HCR™ HiFi Probe: for target RNA1 for use with amplifier X3
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HCR™ HiFi Probe Hybridization Buffer
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HCR™ HiFi Probe Wash Buffer
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HCR™ Gold Amplifier: X3 with label 647
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HCR™ Gold Amplifier Buffer (for use with all kits)
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HCR™ Gold Amplifier Wash Buffer (for use with all kits)
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HCR™ Gold IF kit for target Protein1
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1º antibody: Mouse Anti-Protein1 (your own 1º antibody)
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HCR™ 2º Antibody Probe: Donkey Anti-Mouse for use with amplifier X1
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HCR™ Antibody Buffer​
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HCR™ Gold Amplifier: X1 with label 488​
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