A new era for in situ hybridization:
multiplexed, quantitative, high-resolution, sensitive, versatile, robust
HCR™ RNA-FISH
Multiplexed, quantitative, high-resolution RNA imaging
Multiplexed Experiment
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Order one HCR™ RNA-FISH bundle per target RNA
Example 2-Plex Experiment
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HCR™ RNA-FISH bundle for target mRNA1
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HCR™ probe set: target mRNA1 for use with amplifier B1
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HCR™ amplifier: B1-647
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HCR™ RNA-FISH buffers: probe hybridization buffer, probe wash buffer, amplification buffer (for use with all bundles)
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HCR™ RNA-FISH bundle for target mRNA2
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HCR™ probe set: target mRNA2 for use with amplifier B2
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HCR™ amplifier: B2-488
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The same probe sets and amplifiers enable multiplexed quantitative RNA flow cytometry and northern blotting
Experience multiplexed, quantitative, high-resolution immunfluorescence: amplified, enzyme-free, FFPE, simple
HCR™ IF
Multiplexed, quantitative, high-resolution protein imaging
Multiplexed Experiment
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Order one HCR™ IF bundle per target protein
Example 2-Plex Experiment
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HCR™ IF bundle for target Protein1
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1º Ab: Rabbit Anti-Protein1 (your own 1º antibody)
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HCR™ 2º Ab probe: Donkey Anti-Rabbit for use with amplifier B1
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HCR™ amplifier: B1-647
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HCR™ IF buffers: antibody buffer, amplification buffer (for use with all bundles)
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HCR™ IF bundle for target Protein2
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1º Ab: Mouse Anti-Protein2 (your own 1º antibody)
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HCR™ 2º Ab probe: Donkey Anti-Mouse for use with amplifier B2
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HCR™ amplifier: B2-488
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Quantitative 1-step enzyme-free HCR™ signal amplification for all protein and RNA targets simultaneously
HCR™ IF + HCR™ RNA-FISH
Simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging
Multiplexed Experiment
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Order one HCR™ IF bundle per target protein
-
Order one HCR™ RNA-FISH bundle per target RNA
Example 2-Plex Experiment
-
HCR™ RNA-FISH bundle for target mRNA1
-
HCR™ probe set: target mRNA1 for use with amplifier B1
-
HCR™ amplifier: B1-647
-
HCR™ RNA-FISH buffers: probe hybridization buffer, probe wash buffer, amplification buffer
-
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HCR™ IF bundle for target Protein1
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1º Ab: Mouse Anti-Protein1 (your own 1º antibody)
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HCR™ 2º Ab probe: Donkey Anti-Mouse for use with amplifier B2
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HCR™ amplifier: B2-488
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HCR™ IF buffers: antibody buffer, amplification buffer
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