HCR™ Gold IF
Game-changing multiplex protein imaging
Use the breakthrough HCR™ HiFi Encoder to robustly encode best-in-class quantitative signal amplification to your favorite 1º antibodies for same-species/isotype multiplex immunofluorescence (IF)
​Goodbye IF Multiplexing Tradeoffs! Hello HCR™ HiFi Encoder!
For decades, existing IF products have faced an unfortunate tradeoff – either limiting flexibility through imposition of predetermined panels for technologies that are not robust when applied to users' primaries of choice, or else limiting multiplexing through use of secondaries that prohibit use of same-species/isotype primaries. The HCR™ HiFi Encoder escapes this tradeoff by giving researchers the flexibility to robustly and routinely encode best-in-class signal amplification to their trusted 1º antibodies, enabling same-species/isotype multiplex IF.
What comes in an HCR™ Gold IF kit?
-
HCR™ HiFi Encoder
-
HCR™ HiFi Antibody Buffer
-
HCR™ HiFi Antibody Wash Buffer
-
HCR™ Gold Amplifier
-
HCR™ Gold Amplifier Buffer
-
HCR™ Gold Amplifier Wash Buffer
Simple Robust Protocols
HCR™ Gold IF protocols are simple, robust, and enzyme-free, requiring only 2 stages (antibody binding, amplification) independent of the number of target proteins
Robust Amplification Encoding
Encode your favorite 1º antibodies for use with best-in-class HCR™ Gold Amplifiers (X1, X2, ..., X10) without affecting target-binding affinity.
​​
Same-Species/Isotype Multiplexing
Perform multiplex IF using multiple 1º antibodies from the same host species/isotype (e.g., mouse IgG1 or rabbit IgG) with no cross-reactitivity. For example, perform 10-Plex HCR™ Gold IF using rabbit IgG 1º antibodies for all 10 target proteins.
Best-in-Class Amplification
Use 1-step quantitative HCR™ Gold signal amplification for protein imaging, leveraging the dynamic nanotechnology that has redefined the state-of-the-art for RNA imaging. ​​​
Quantitative High-Resolution Protein Imaging
HCR™ Gold IF enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.
1-step quantitative HCR™ Gold signal amplification for all target proteins simultaneously
Protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples
Getting started?
For game-changing multiplex protein imaging using HCR™ Gold IF:
​
✓ use your own favorite 1º antibodies (same host species/isotype for each target protein if desired)
✓ order one HCR™ Gold IF kit per target protein (select a different HCR™ HiFi Encoder and HCR™ Gold Amplifier for each target protein)