HCR v3.0 Technologies

HCR v3.0 enables multiplexed quantitative RNA fluorescence in situ hybridization (RNA-FISH), RNA flow cytometry, and northern blotting with automatic background suppression throughout the protocol, dramatically enhancing performance and ease-of-use.

HCRv3-insituhybridization-thumbnail-01.p

A new era for in situ hybridization: 

multiplexed, quantitative, sensitive,

versatile, robust

HCR v3.0

HCR-multiplexing-thumbnail-01.png

The same 2-stage enzyme-free protocol is used independent of the number of target RNAs

Multiplexing

HCR-readout-readin-thumbnail-01.png

Bidirectional quantitative RNA discovery in an anatomical context

Read-Out/Read-In

HCR-hairpins-thumbnail-01.png

Multiplexed, quantitative, isothermal, enzyme-free signal amplification

Hybridization Chain Reaction (HCR)

qHCR-imaging-thumbnail-01.png

Analog RNA relative quantitation with subcellular resolution in the anatomical context of thick autofluorescent samples

qHCR Imaging

qHCR-flowcytometry-thumbnail-01.png

Analog RNA relative quantitation for high-throughput expression profiling of mammalian and bacterial cells

qHCR Flow Cytometry 

HCRv3-automatic-background-suppression-0

Reagents will not generate amplified background even if they bind non-specifically within the sample

Automatic Background Suppression

dHCR-imaging-thumbnail-01.png

Digital RNA absolute quantitation with single-molecule resolution in the anatomical context of thick autofluorescent samples

dHCR Imaging

qHCR-northernblot-thumbnail-01.png

Simultaneous quantitation of

RNA target size and abundance

qHCR Northern Blots 

We recently updated our Privacy Policy to include new sections on Google Analytics and Do Not Track.

Please visit our Privacy Policy to see the full details.

Copyright © 2019 Molecular Instruments, Inc. All rights reserved.