HCR v3.0 Technologies

HCR v3.0 enables multiplexed quantitative RNA fluorescence in situ hybridization (RNA-FISH), RNA flow cytometry, and northern blotting with automatic background suppression throughout the protocol, dramatically enhancing performance and ease-of-use.

HCRv3-insituhybridization-thumbnail-01.p

HCR v3.0

A new era for in situ hybridization: 

multiplexed, quantitative, sensitive,

versatile, robust

HCR-multiplexing-thumbnail-01.png

Multiplexing

The same 2-stage enzyme-free protocol is used independent of the number of target RNAs

HCR-readout-readin-thumbnail-01.png

Read-Out/Read-In

Bidirectional quantitative RNA discovery in an anatomical context

HCR-hairpins-thumbnail-01.png

Hybridization Chain Reaction (HCR)

Multiplexed, quantitative, isothermal, enzyme-free signal amplification

qHCR-imaging-thumbnail-01.png

qHCR Imaging

Analog RNA relative quantitation with subcellular resolution in the anatomical context of thick autofluorescent samples

qHCR-flowcytometry-thumbnail-01.png

qHCR Flow Cytometry 

Analog RNA relative quantitation for high-throughput expression profiling of mammalian and bacterial cells

HCRv3-automatic-background-suppression-0

Automatic Background Suppression

Reagents will not generate amplified background even if they bind non-specifically within the sample

dHCR-imaging-thumbnail-01.png

dHCR Imaging

Digital RNA absolute quantitation with single-molecule resolution in the anatomical context of thick autofluorescent samples

qHCR-northernblot-thumbnail-01.png

qHCR Northern Blots 

Simultaneous quantitation of

RNA target size and abundance