HCR v3.0 Technologies
HCR v3.0 enables multiplexed quantitative RNA fluorescence in situ hybridization (RNA-FISH), RNA flow cytometry, and northern blotting with automatic background suppression throughout the protocol, dramatically enhancing performance and ease-of-use.
HCR v3.0
A new era for in situ hybridization:
multiplexed, quantitative, sensitive,
versatile, robust
Multiplexing
The same 2-stage enzyme-free protocol is used independent of the number of target RNAs
Read-Out/Read-In
Bidirectional quantitative RNA discovery in an anatomical context
Hybridization Chain Reaction (HCR)
Multiplexed, quantitative, isothermal, enzyme-free signal amplification
qHCR Imaging
Analog RNA relative quantitation with subcellular resolution in the anatomical context of thick autofluorescent samples
qHCR Flow Cytometry
Analog RNA relative quantitation for high-throughput expression profiling of mammalian and bacterial cells
Automatic Background Suppression
Reagents will not generate amplified background even if they bind non-specifically within the sample
dHCR Imaging
Digital RNA absolute quantitation with single-molecule resolution in the anatomical context of thick autofluorescent samples
qHCR Northern Blots
Simultaneous quantitation of
RNA target size and abundance