HCR v3.0 Technologies

HCR v3.0 enables multiplexed quantitative RNA fluorescence in situ hybridization (RNA-FISH), RNA flow cytometry, and northern blotting with automatic background suppression throughout the protocol, dramatically enhancing performance and ease-of-use.

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HCR v3.0

A new era for in situ hybridization:

multiplexed, quantitative, sensitive,

versatile, robust

Multiplexing

The same 2-stage enzyme-free protocol is used independent of the number of target RNAs

Read-Out/Read-In

Bidirectional quantitative RNA discovery in an anatomical context

Hybridization Chain Reaction (HCR)

Multiplexed, quantitative, isothermal, enzyme-free signal amplification

qHCR Imaging

Analog RNA relative quantitation with subcellular resolution in the anatomical context of thick autofluorescent samples

qHCR Flow Cytometry

Analog RNA relative quantitation for high-throughput expression profiling of mammalian and bacterial cells

Automatic Background Suppression

Reagents will not generate amplified background even if they bind non-specifically within the sample

dHCR Imaging

Digital RNA absolute quantitation with single-molecule resolution in the anatomical context of thick autofluorescent samples

qHCR Northern Blots

Simultaneous quantitation of

RNA target size and abundance