Billing & Shipping FAQ

 

How do I order and pay for HCR bundles?

  • Place an order for one or more bundles at the Molecular Instruments® online store.

  • You will receive an email quote which you can use to upload your purchase order.

  • Your institution will receive an email invoice at time of shipment.

  • Payment is due by ACH or wire transfer within 30 days of the invoice date. 

Can I cancel my order? 

Yes! You can cancel your order up until the time your purchase order is accepted. Please contact the Molecular Instruments Team (support@molecularinstruments.com).

 

Do you offer academic discounts? 

Yes! If your organization is a university, government lab, or non-profit research institute, you are eligible for academic pricing. Register to see applicable discounts. 

 

What are your estimated shipping times? 

Upon receiving a valid purchase order, we aim to ship as follows:

  • HCR RNA-FISH probe set: 1 business day

  • HCR IHC probe: 1 business day

  • HCR amplifier: 1 business day

  • HCR buffers: 1 business day

  • Custom HCR RNA-FISH probe set: 5 business days (increased to 8 during COVID) 

  • Custom-labeled HCR amplifier: 10 business days (increased to 12 during COVID) 

Ordering HCR RNA-FISH Reagents FAQ

 

What comes in an HCR RNA-FISH bundle?

  • HCR probe set (designed by Molecular Instruments Team)

  • HCR amplifier (QC-tested)

  • HCR buffers: probe hybridization buffer, probe wash buffer, amplification buffer

 

Can I order a subset of these components?
Yes! You can order bundles containing any subset of the above components. 

What do I order for a multiplexed HCR RNA-FISH experiment?

  • Order one probe set for each target RNA.

  • Order one HCR amplifier per target (for example, amplifier B1 for target 1, amplifier B2 for target 2, …); choose a different fluorophore (647, 594, …) for each HCR amplifier that will be imaged in the same sample (for example, B1-647, B2-594, …).

  • Order HCR RNA-FISH buffers. 

  • Example 3-plex experiment: 

    • HCR bundle for target mRNA1:

      • HCR probe set: target mRNA1 for use with B1

      • HCR amplifier: B1-647

      • HCR RNA-FISH buffers (for use with all bundles) 

    • HCR bundle for target mRNA2:​

      • HCR probe set: target mRNA2 for use with B2

      • HCR amplifier: B2-546​

    • HCR bundle for target mRNA3:​

      • HCR probe set: target mRNA3 for use with B3 

      • HCR amplifier: B3-488

What probe set size do you recommend for detecting RNA targets?

  • qHCR RNA imaging: 20+ split-initiator probe pairs per target RNA (as permitted by target length); signal-to-background and precision increase with probe set size.

  • dHCR RNA imaging: 30+ split-initiator probe pairs per target RNA (maximize probe set size based on length of target); fidelity increases with probe set size.

  • qHCR RNA flow cytometry: 30+ split-initiator probe pairs per target RNA (maximize probe set size based on length of target); signal-to-background and precision increase with probe set size.

  • qHCR northern blotting: 20+ split-initiator probe pairs per target RNA (as permitted by target length); signal-to-background and precision increase with probe set size.

 

I've never used HCR RNA-FISH — what’s the best way to get started?
Please see our HCR RNA-FISH protocols, which provide a starting point for working in diverse organisms. Start with a positive control probe set if one is available for your sample type (marked with a check mark in the Probe Set dropdown). Otherwise, order a custom probe set for a high- or moderate-copy target.

I am getting great results with an HCR probe set that I previously ordered from moleculartechnologies.org — can I order more of the exact same probe set from Molecular Instruments®? 

Yes! Please include a note in the Comment field of your order (include your previous Order # if available).

Can I order an HCR amplifier labeled with a custom fluorophore? 

Yes. Choose Custom in the Amplifier Label pulldown and enter the name of the fluorophore (currently 350, 405, 430, 532, 555, 568, 660, 680, 700, 750, 790, or Cyanine 5.5 or 7). Custom labeling requires a minimum scale of 5 nmol. The 5 nmol can be spread across up to three HCR amplifiers (e.g., B1-Cyanine 7, B2-Cyanine 7, B3-Cyanine 7), with a minimum of 1 nmol per amplifier (include a comment describing your custom label order in the Comment field). 

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Ordering HCR IHC Reagents FAQ

 

What comes in an HCR IHC bundle?

  • HCR 2º antibody probe (QC-tested)

  • HCR amplifier (QC-tested)

  • HCR buffers: antibody buffer, amplification buffer

Can I order a subset of these components?
Yes! You can order bundles containing any subset of the above components. 

What do I order for a multiplexed HCR IHC experiment?

  • Use your own 1º antibody of choice for each target protein (each 1º antibody raised in a different host organism).

  • Order one HCR 2º antibody probe per target (each 2º antibody probe for use with a different 1º antibody and HCR amplifier).

  • Order one HCR amplifier per target (for example, amplifier B1 for target 1, amplifier B2 for target 2, …); choose a different fluorophore (647, 594, …) for each HCR amplifier that will be imaged in the same sample (for example, B1-647, B2-594, …).

  • Order HCR IHC buffers. 

  • Example 3-plex experiment: 

    • HCR bundle for target Protein1:

      • 1º Ab: Rabbit Anti-Protein1 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Rabbit for use with B1

      • HCR amplifier: B1-647

      • HCR IHC buffers (for use with all bundles) 

    • HCR bundle for target Protein2:

      • 1º Ab: Mouse Anti-Protein2 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Mouse for use with B2

      • HCR amplifier: B2-546​

    • HCR bundle for target Protein3:

      • 1º Ab: Rat Anti-Protein3 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Rat for use with B3 

      • HCR amplifier: B3-488

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Ordering HCR IHC + HCR RNA-FISH Reagents FAQ

 

What do I order for a multiplexed HCR IHC experiment?

  • Order one HCR IHC bundle per target protein (see above for details). 

  • Order one HCR RNA-FISH target per target RNA (see above for details). 

  • Example 4-plex experiment (2 proteins, 2 mRNAs): 

    • HCR bundle for target Protein1:

      • 1º Ab: Rabbit Anti-Protein1 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Rabbit for use with B1

      • HCR amplifier: B1-488

      • HCR IHC buffers (for use with all IHC bundles) 

    • HCR bundle for target Protein2:

      • 1º Ab: Mouse Anti-Protein2 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Mouse for use with B2

      • HCR amplifier: B2-546

    • HCR bundle for target mRNA3:

      • HCR probe set: target mRNA3 for use with B3

      • HCR amplifier: B3-594

      • HCR RNA-FISH buffers (for use with all RNA-FISH bundles) 

    • HCR bundle for target mRNA4:​

      • HCR probe set: target mRNA4 for use with B4

      • HCR amplifier: B4-647

Can my existing HCR RNA-FISH probe sets and amplifiers be used in combination with new HCR IHC bundles to perform simultaneous protein and RNA imaging using HCR IHC + HCR RNA-FISH?

Yes! Just make sure to use a different HCR amplifier and fluorophore (for example, B1-647, B2-594, B3-546, ...) for each target protein or RNA in a multiplexed experiment. 

Are HCR amplifiers interchangeable between HCR RNA-FISH bundles and HCR IHC bundles?

Yes! The amplifiers (B1, B2, ...) are interchangeable between HCR RNA-FISH and HCR IHC bundles. Likewise, HCR Amplification Buffer is interchangeable between HCR RNA-FISH and HCR IHC bundles. 

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Molecular Instruments FAQ

 

Should I share my images with the MI Team?
Yes! We are always excited to see images from your research! 

What if I have other questions? 
Please contact the Molecular Instruments Team (support@molecularinstruments.com).