top of page

Billing & Shipping FAQ

 

How do I order and pay?

  • Place an order for one or more bundles at the Molecular Instruments® online store.

  • You will receive an email quote which you can use to upload your purchase order.

  • Your institution will receive an email invoice at time of shipment.

  • Payment is due by ACH or wire transfer within 30 days of the invoice date. 

Can I cancel my order? 

Yes! You can cancel your order up until the time your purchase order is accepted. Please contact the Molecular Instruments Team (support@molecularinstruments.com).

 

Do you offer academic discounts? 

Yes! If your organization is a university, government lab, or non-profit research institute, you are eligible for academic pricing. Register to see applicable discounts. 

 

What are your estimated shipping times? 

Upon receiving a valid purchase order, we aim to ship as follows:

  • HCR™ IF probe: 1-5 business days

  • HCR™ amplifier: 1-5 business days

  • HCR™ buffers: 1-5 business days

  • HCR™ RNA-FISH probe set: 5-15 business days 

  • Custom HCR™ amplifier: please contact us

Ordering HCR™ RNA-FISH Reagents FAQ

 

What comes in an HCR™ RNA-FISH bundle?

  • HCR™ probe set (designed by Molecular Instruments Team)

  • HCR™ amplifier (QC-tested)

  • HCR™ buffers: probe hybridization buffer, probe wash buffer, amplification buffer

 

Can I order a subset of these components?
Yes! You can order bundles containing any subset of the above components. 

What do I order for a multiplexed HCR™ RNA-FISH experiment?

  • Order one HCR™ probe set for each target RNA.

  • Order one HCR™ amplifier per target (for example, amplifier B1 for target 1, amplifier B2 for target 2, …); choose a different fluorophore (647, 594, …) for each amplifier that will be imaged in the same sample (for example, B1-647, B2-594, …).

  • Order HCR™ RNA-FISH buffers. 

  • Example 3-plex experiment: 

    • HCR™ RNA-FISH bundle for target mRNA1:

      • HCR™ probe set: target mRNA1 for use with B1

      • HCR™ amplifier: B1-647

      • HCR™ RNA-FISH buffers (for use with all bundles) 

    • HCR™ RNA-FISH bundle for target mRNA2:​

      • HCR™ probe set: target mRNA2 for use with B2

      • HCR™ amplifier: B2-546​

    • HCR™ RNA-FISH bundle for target mRNA3:​

      • HCR™ probe set: target mRNA3 for use with B3 

      • HCR™ amplifier: B3-488

What probe set size do you recommend for detecting RNA targets?

  • Subcellular quantitative RNA imaging: 20+ split-initiator probe pairs per target RNA (as permitted by target length); signal-to-background and precision increase with probe set size.

  • Single-molecule quantitative RNA imaging: 30+ split-initiator probe pairs per target RNA (maximize probe set size based on length of target); fidelity increases with probe set size.

  • Quantitative RNA flow cytometry: 30+ split-initiator probe pairs per target RNA (maximize probe set size based on length of target); signal-to-background and precision increase with probe set size.

  • Quantitative northern blotting: 20+ split-initiator probe pairs per target RNA (as permitted by target length); signal-to-background and precision increase with probe set size.

 

I've never used HCR™ RNA-FISH — what’s the best way to get started?
Please see our HCR™ RNA-FISH protocols, which provide a starting point for working in diverse organisms. Start with a positive control probe set if one is available for your sample type (marked with a check mark in the Probe Set dropdown). Otherwise, order a custom probe set for a high- or moderate-copy target.

Can I order an HCR™ amplifier labeled with a custom fluorophore? 

Yes. Choose Custom in the Amplifier Label pulldown and enter the name of the fluorophore (currently 350, 405, 430, 532, 555, 568, 660, 680, 700, 750, 790, or Cyanine 5.5 or 7). Custom labeling requires a minimum scale of 5 nmol. The 5 nmol can be spread across up to three HCR™ amplifiers (e.g., B1-Cyanine 7, B2-Cyanine 7, B3-Cyanine 7), with a minimum of 1 nmol per amplifier (include a comment describing your custom label order in the Comment field). 

logos_RNA.png

Ordering HCR™ IF Reagents FAQ

 

What comes in an HCR™ IF bundle?

  • HCR™ 2º antibody probe (QC-tested)

  • HCR™ amplifier (QC-tested)

  • HCR™ buffers: antibody buffer, amplification buffer

Can I order a subset of these components?
Yes! You can order bundles containing any subset of the above components. 

What do I order for a multiplexed HCR™ IF experiment?

  • Use your own 1º antibody of choice for each target protein (each 1º antibody raised in a different host organism).

  • Order one HCR™ 2º antibody probe per target (each 2º antibody probe for use with a different 1º antibody and amplifier).

  • Order one HCR™ amplifier per target (for example, amplifier B1 for target 1, amplifier B2 for target 2, …); choose a different fluorophore (647, 594, …) for each amplifier that will be imaged in the same sample (for example, B1-647, B2-594, …).

  • Order HCR™ IF buffers. 

  • Example 3-plex experiment: 

    • HCR™ IF bundle for target Protein1:

      • 1º Ab: Rabbit Anti-Protein1 (your own 1º antibody) 

      • HCR™ 2º Ab ​probe: Donkey Anti-Rabbit for use with B1

      • HCR™ amplifier: B1-647

      • HCR™ IF buffers (for use with all bundles) 

    • HCR™ IF bundle for target Protein2:

      • 1º Ab: Mouse Anti-Protein2 (your own 1º antibody) 

      • HCR™ 2º Ab ​probe: Donkey Anti-Mouse for use with B2

      • HCR™ amplifier: B2-546​

    • HCR™ IF bundle for target Protein3:

      • 1º Ab: Rat Anti-Protein3 (your own 1º antibody) 

      • HCR™ 2º Ab ​probe: Donkey Anti-Rat for use with B3 

      • HCR™ amplifier: B3-488

logos_Protein.png

Ordering HCR™ IF + HCR™ RNA-FISH Reagents FAQ

 

What do I order for a multiplexed HCR™ IF experiment?

  • Order one HCR™ IF bundle per target protein (see above for details). 

  • Order one HCR™ RNA-FISH target per target RNA (see above for details). 

  • Example 4-plex experiment (2 proteins, 2 mRNAs): 

    • HCR™ IF bundle for target Protein1:

      • 1º Ab: Rabbit Anti-Protein1 (your own 1º antibody) 

      • HCR™ 2º Ab ​probe: Donkey Anti-Rabbit for use with B1

      • HCR™ amplifier: B1-488

      • HCR™ IF buffers (for use with all IF bundles) 

    • HCR™ IF bundle for target Protein2:

      • 1º Ab: Mouse Anti-Protein2 (your own 1º antibody) 

      • HCR™ 2º Ab ​probe: Donkey Anti-Mouse for use with B2

      • HCR™ amplifier: B2-546

    • HCR™ RNA-FISH bundle for target mRNA3:

      • HCR™ probe set: target mRNA3 for use with B3

      • HCR™ amplifier: B3-594

      • HCR™ RNA-FISH buffers (for use with all RNA-FISH bundles) 

    • HCR™ RNA-FISH bundle for target mRNA4:​

      • HCR™ probe set: target mRNA4 for use with B4

      • HCR™ amplifier: B4-647

Can my existing HCR™ RNA-FISH probe sets and amplifiers be used in combination with new HCR™ IF bundles to perform simultaneous protein and RNA imaging using HCR™ IF + HCR™ RNA-FISH?

Yes! Just make sure to use a different HCR™ amplifier and fluorophore (for example, B1-647, B2-594, B3-546, ...) for each target protein or RNA in a multiplexed experiment. 

Are HCR™ amplifiers interchangeable between HCR™ RNA-FISH bundles and HCR™ IF bundles?

Yes! The amplifiers (B1, B2, ...) are interchangeable between HCR™ RNA-FISH and HCR™ IF bundles. Likewise, HCR™ Amplification Buffer is interchangeable between HCR™ RNA-FISH and HCR™ IF bundles. 

logos_Protein.png
logos_RNA.png

Molecular Instruments FAQ

 

Should I share my images with the MI Team?
Yes! We are always excited to see images from your research! 

What if I have other questions? 
Please contact the Molecular Instruments Team (support@molecularinstruments.com). 

All major 

automation platforms 

hcr-tm-automated-white-01.png
bottom of page