Billing & Shipping FAQ

 

How do I order and pay for HCR bundles?

  • Place an order for one or more bundles at the Molecular Instruments® online store.

  • You will receive an email quote which you can use to upload your purchase order.

  • Your institution will receive an email invoice at time of shipment.

  • Payment is due by ACH or wire transfer within 30 days of the invoice date. 

Can I cancel my order? 

Yes! You can cancel your order up until the time your purchase order is accepted. Please contact the Molecular Instruments Team (support@molecularinstruments.com).

 

Do you offer academic discounts? 

Yes! If your organization is a university, government lab, or non-profit research institute, you are eligible for academic pricing. Register to see applicable discounts. 

 

What are your estimated shipping times? 

Upon receiving a valid purchase order, we aim to ship as follows:

  • HCR RNA-FISH probe set: 1 business day

  • HCR IHC probe: 1 business day

  • HCR amplifier: 1 business day

  • HCR buffers: 1 business day

  • Custom HCR RNA-FISH probe set: 5 business days (increased to 8 during COVID) 

  • Custom-labeled HCR amplifier: 10 business days (increased to 12 during COVID) 

Ordering HCR RNA-FISH Reagents FAQ

 

What comes in an HCR RNA-FISH bundle?

  • HCR probe set (designed by Molecular Instruments Team)

  • HCR amplifier (QC-tested)

  • HCR buffers: probe hybridization buffer, probe wash buffer, amplification buffer

 

Can I order a subset of these components?
Yes! You can order bundles containing any subset of the above components. 

What do I order for a multiplexed HCR RNA-FISH experiment?

  • Order one probe set for each target RNA.

  • Order one HCR amplifier per target (for example, amplifier B1 for target 1, amplifier B2 for target 2, …); choose a different fluorophore (Alexa Fluor® 647, Alexa Fluor® 594, …) for each HCR amplifier that will be imaged in the same sample (for example, B1-647, B2-594, …).

  • Order HCR RNA-FISH buffers. 

  • Example 3-plex experiment: 

    • HCR bundle for target mRNA1:

      • HCR probe set: target mRNA1 for use with B1

      • HCR amplifier: B1-647

      • HCR RNA-FISH buffers (for use with all bundles) 

    • HCR bundle for target mRNA2:​

      • HCR probe set: target mRNA2 for use with B2

      • HCR amplifier: B2-546​

    • HCR bundle for target mRNA3:​

      • HCR probe set: target mRNA3 for use with B3 

      • HCR amplifier: B3-488

What probe set size do you recommend for detecting RNA targets?

  • qHCR RNA imaging: 20+ split-initiator probe pairs per target RNA (as permitted by target length); signal-to-background and precision increase with probe set size.

  • dHCR RNA imaging: 30+ split-initiator probe pairs per target RNA (maximize probe set size based on length of target); fidelity increases with probe set size.

  • qHCR RNA flow cytometry: 30+ split-initiator probe pairs per target RNA (maximize probe set size based on length of target); signal-to-background and precision increase with probe set size.

  • qHCR northern blotting: 20+ split-initiator probe pairs per target RNA (as permitted by target length); signal-to-background and precision increase with probe set size.

 

I've never used HCR RNA-FISH — what’s the best way to get started?
Please see our HCR RNA-FISH protocols, which provide a starting point for working in diverse organisms. Start with a positive control probe set if one is available for your sample type (marked with a check mark in the Probe Set dropdown). Otherwise, order a custom probe set for a high- or moderate-copy target.

I am getting great results with an HCR probe set that I previously ordered from moleculartechnologies.org — can I order more of the exact same probe set from Molecular Instruments®? 

Yes! Please include a note in the Comment field of your order (include your previous Order # if available).

Can I order an HCR amplifier labeled with a custom fluorophore? 

Yes. Choose Custom in the Amplifier Label pulldown and enter the name of the fluorophore (currently any of the Alex Fluor® dyes or Cyanine 7 or 7.5). Custom labeling requires a minimum scale of 5 nmol. The 5 nmol can be spread across up to three HCR amplifiers (e.g., B1-Cyanine 7, B2-Cyanine 7, B3-Cyanine 7), with a minimum of 1 nmol per amplifier (include a comment describing your custom label order in the Comment field). 

logos_RNA.png

Ordering HCR IHC Reagents FAQ

 

What comes in an HCR IHC bundle?

  • HCR 2º antibody probe (QC-tested)

  • HCR amplifier (QC-tested)

  • HCR buffers: antibody buffer, amplification buffer

Can I order a subset of these components?
Yes! You can order bundles containing any subset of the above components. 

What do I order for a multiplexed HCR IHC experiment?

  • Use your own 1º antibody of choice for each target protein (each 1º antibody raised in a different host organism).

  • Order one HCR 2º antibody probe per target (each 2º antibody probe for use with a different 1º antibody and HCR amplifier).

  • Order one HCR amplifier per target (for example, amplifier B1 for target 1, amplifier B2 for target 2, …); choose a different fluorophore (Alexa Fluor® 647, Alexa Fluor® 594, …) for each HCR amplifier that will be imaged in the same sample (for example, B1-647, B2-594, …).

  • Order HCR IHC buffers. 

  • Example 3-plex experiment: 

    • HCR bundle for target Protein1:

      • 1º Ab: Rabbit Anti-Protein1 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Rabbit for use with B1

      • HCR amplifier: B1-647

      • HCR IHC buffers (for use with all bundles) 

    • HCR bundle for target Protein2:

      • 1º Ab: Mouse Anti-Protein2 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Mouse for use with B2

      • HCR amplifier: B2-546​

    • HCR bundle for target Protein3:

      • 1º Ab: Rat Anti-Protein3 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Rat for use with B3 

      • HCR amplifier: B3-488

logos_Protein.png

Ordering HCR IHC + HCR RNA-FISH Reagents FAQ

 

What do I order for a multiplexed HCR IHC experiment?

  • Order one HCR IHC bundle per target protein (see above for details). 

  • Order one HCR RNA-FISH target per target RNA (see above for details). 

  • Example 4-plex experiment (2 proteins, 2 mRNAs): 

    • HCR bundle for target Protein1:

      • 1º Ab: Rabbit Anti-Protein1 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Rabbit for use with B1

      • HCR amplifier: B1-488

      • HCR IHC buffers (for use with all IHC bundles) 

    • HCR bundle for target Protein2:

      • 1º Ab: Mouse Anti-Protein2 (your own 1º antibody) 

      • HCR 2º Ab ​probe: Donkey Anti-Mouse for use with B2

      • HCR amplifier: B2-546

    • HCR bundle for target mRNA3:

      • HCR probe set: target mRNA3 for use with B3

      • HCR amplifier: B3-594

      • HCR RNA-FISH buffers (for use with all RNA-FISH bundles) 

    • HCR bundle for target mRNA4:​

      • HCR probe set: target mRNA4 for use with B4

      • HCR amplifier: B4-647

Can my existing HCR RNA-FISH probe sets and amplifiers be used in combination with new HCR IHC bundles to perform simultaneous protein and RNA imaging using HCR IHC + HCR RNA-FISH?

Yes! Just make sure to use a different HCR amplifier and fluorophore (for example, B1-647, B2-594, B3-546, ...) for each target protein or RNA in a multiplexed experiment. 

Are HCR amplifiers interchangeable between HCR RNA-FISH bundles and HCR IHC bundles?

Yes! The amplifiers (B1, B2, ...) are interchangeable between HCR RNA-FISH and HCR IHC bundles. Likewise, HCR Amplification Buffer is interchangeable between HCR RNA-FISH and HCR IHC bundles. 

logos_Protein.png
logos_RNA.png

Molecular Instruments FAQ

 

Should I share my images with the MI Team?
Yes! We are always excited to see images from your research! 

What if I have other questions? 
Please contact the Molecular Instruments Team (support@molecularinstruments.com).