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HCR RNA-FISH: Protocol Overview
The same 2-stage protocol is used independent of the number of target RNAs.
Whole-mount red flour beetle embryos
Tribolium castaneum
HCR Image Gallery
Channels:
DAPI toll10 wg + sim
mlpt gt kni
cas toll10 wg nub
sog wg twi sim
toll10 wg + sim Ubx Abd-A Abd-B
HCR RNA-FISH
Embryos fixed: 8 h to 3 d after egg lay
Image credit: O. Tidswell
nub toll10 stg cas
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