HCR™ Gold RNA Flow Cytometry
Multiplex quantitative RNA flow cytometry
HCR™ Gold RNA-FISH kits enable multiplex quantitative RNA flow cytometry with automatic background suppression throughout the protocol, enabling high-throughput RNA expression profiling of mammalian cells and bacteria without the need to engineer reporter lines
Simple Robust Protocols
HCR™ Gold RNA Flow Cytometry protocols are simple, robust, and enzyme-free, requiring only 2 stages (probe hybridization, amplification) independent of the number of target RNAs
Quantitative RNA Flow Cytometry in Mammalian Cells and Bacteria
Redundant 2-Channel Detection
To illustrate the quantitative nature of HCR™ RNA flow cytometry, we detect a target mRNA using two probe sets that trigger different amplifiers carrying spectrally distinct fluorophores.
Mammalian Cells (HEK 293T)
Bacteria (E. coli)
High Accuracy and Precision
The resulting scatter plots of single-cell signal demonstrate high accuracy (linearity with zero intercept) and precision (tight scatter around the line) in mammalian cells and bacteria.
Automatic Background Suppression
HCR™ Gold RNA Flow Cytometry provides automatic background suppression throughout the protocol, ensuring that even if probes or amplifiers bind non-specifically in the sample they will not generate amplified background, dramatically enhancing performance and ease-of-use.
Straightforward Multiplexing
HCR™ Gold RNA Flow Cytometry enables straightforward multiplexing using 1-step quantitative signal amplification for all target RNAs simultaneously.
Infinite Catalog
Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.
Quantitative Signal
HCR™ Gold RNA Flow Cytometry signal is quantitative in the form of single-cell fluorescence intensities that scale approximately linearly with the number of RNA target molecules per cell.
Precision Increases with Target Binding Sites
Quantitative precision increases with the number of target binding sites due to the benefits of automatic background suppression. For quantitative HCR™ Gold RNA Flow Cytometry, we recommend using 30+ binding sites per target RNA (maximize given target length).