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Straightforward Multiplexing

HCR™ amplifiers enable straightforward multiplexing using: 

by providing quantitative, 1-step, high-gain, enzyme-free signal amplification for all protein and/or RNA targets simultaneously.

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Orthogonal Amplifiers

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Orthogonal amplifiers carrying spectrally distinct fluorophores operate independently in the same sample at the same time.

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HCR™ RNA-FISH

A new era for in situ hybridization: 

multiplexed, quantitative, high-resolution, sensitive, versatile, robust

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HCR™ IF

Experience multiplexed, quantitative, high-resolution immunfluorescence: amplified, enzyme-free, FFPE, simple

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HCR™ IF + HCR™ RNA-FISH

Quantitative 1-step enzyme-free HCR™ signal amplification for all protein and RNA targets simultaneously

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Catalog and Custom HCR™ Amplifiers

We offer a catalog of 5 orthogonal amplifiers labeled with any of 5 fluorophores (488, 514, 546, 594, 647). A total of 10 orthogonal amplifiers are available for custom labeling with fluorophores including 350, 405, 430, 532, 555, 568, 660, 680, 700, 750, 790, or Cyanine 5.5 or 7. 

Straightforward Multiplexing

✓ Orthogonal HCR™ probes

✓ Orthogonal HCR™ amplifiers

✓ Simple 2-stage enzyme-free protocols for IF, RNA-FISH, RNA flow cytometry, and northern blots

✓ Simple 3-stage enzyme-free protocols for IF + RNA-FISH

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