Straightforward Multiplexing

HCR amplifiers enable straightforward multiplexing using: 

by providing quantitative, 1-step, high-gain, enzyme-free signal amplification for all protein and/or RNA targets simultaneously.

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Orthogonal HCR Amplifiers

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Orthogonal HCR amplifiers carrying spectrally distinct fluorophores operate independently in the same sample at the same time.

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A new era for in situ hybridization: 

multiplexed, quantitative, high-resolution, sensitive, versatile, robust

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Experience multiplexed, quantitative, high-resolution immunhistochemistry: amplified, enzyme-free, FFPE, simple

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Quantitative 1-step enzyme-free HCR signal amplification for all protein and RNA targets simultaneously

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Catalog and Custom HCR Amplifiers

We offer a catalog of 5 orthogonal HCR amplifiers labeled with any of 5 Alexa Fluor® dyes. HCR amplifiers can also be custom-labeled with any of the other Alexa Fluor® dyes or Cyanine 5.5 or 7. 

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Simultaneous Quantitative High-Resolution Protein and RNA Imaging

qHCR protein and RNA imaging enables protein and RNA relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

Straightforward Multiplexing

 Orthogonal HCR probes

Orthogonal HCR amplifiers

Simple 2-stage enzyme-free protocols for IHC, RNA-FISH, RNA flow cytometry, and northern blots

Simple 3-stage enzyme-free protocols for IHC + RNA-FISH