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Straightforward Multiplexing

HCR amplifiers enable straightforward multiplexing using: 

by providing quantitative, 1-step, high-gain, enzyme-free signal amplification for all protein and/or RNA targets simultaneously.


Orthogonal HCR Amplifiers


Orthogonal HCR amplifiers carrying spectrally distinct fluorophores operate independently in the same sample at the same time.


A new era for in situ hybridization: 

multiplexed, quantitative, high-resolution, sensitive, versatile, robust


Experience multiplexed, quantitative, high-resolution immunhistochemistry: amplified, enzyme-free, FFPE, simple


Quantitative 1-step enzyme-free HCR signal amplification for all protein and RNA targets simultaneously


Catalog and Custom HCR Amplifiers

We offer a catalog of 5 orthogonal HCR amplifiers labeled with any of 5 fluorophores (488, 514, 546, 594, 647). HCR amplifiers can also be custom-labeled with other fluorophores including 350, 405, 430, 532, 555, 568, 660, 680, 700, 750, 790, or Cyanine 5.5 or 7. 


Simultaneous Quantitative High-Resolution Protein and RNA Imaging

qHCR protein and RNA imaging enables protein and RNA relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

Straightforward Multiplexing

 Orthogonal HCR probes

Orthogonal HCR amplifiers

Simple 2-stage enzyme-free protocols for IHC, RNA-FISH, RNA flow cytometry, and northern blots

Simple 3-stage enzyme-free protocols for IHC + RNA-FISH

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