HCR RNA-FISH: Protocol Overview
The same 2-stage protocol is used independent of the number of target RNAs.
Straightforward Multiplexing
HCR amplifiers enable straightforward multiplexing using:
by providing quantitative, 1-step, high-gain, enzyme-free signal amplification for all protein and/or RNA targets simultaneously.
Orthogonal HCR Amplifiers
Orthogonal HCR amplifiers carrying spectrally distinct fluorophores operate independently in the same sample at the same time.
A new era for in situ hybridization:
multiplexed, quantitative, high-resolution, sensitive, versatile, robust
Experience multiplexed, quantitative, high-resolution immunhistochemistry: amplified, enzyme-free, FFPE, simple
Quantitative 1-step enzyme-free HCR signal amplification for all protein and RNA targets simultaneously
Catalog and Custom HCR Amplifiers
We offer a catalog of 5 orthogonal HCR amplifiers labeled with any of 5 fluorophores (488, 514, 546, 594, 647). HCR amplifiers can also be custom-labeled with other fluorophores including 350, 405, 430, 532, 555, 568, 660, 680, 700, 750, 790, or Cyanine 5.5 or 7.
Simultaneous Quantitative High-Resolution Protein and RNA Imaging
qHCR protein and RNA imaging enables protein and RNA relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.
Straightforward Multiplexing
✓ Orthogonal HCR probes
✓ Orthogonal HCR amplifiers
✓ Simple 2-stage enzyme-free protocols for IHC, RNA-FISH, RNA flow cytometry, and northern blots
✓ Simple 3-stage enzyme-free protocols for IHC + RNA-FISH