HCR™ Gold RNA-FISH/2ºIF
Unified multiplex, quantitative, high-resolution RNA and protein imaging
Combine HCR™ Gold RNA-FISH and HCR™ Gold 2ºIF (must use a different host species/isotype for each target protein) with 1-step quantitative signal amplification for 10 RNA and/or protein targets simultaneously
Simple Robust Protocols
HCR™ Gold RNA-FISH/2ºIF protocols are simple, robust, and enzyme-free, requiring only 3 stages (probe binding to protein targets, probe hybridization to RNA targets, amplification) independent of the number of RNA and protein targets
Straightforward Multiplexing
HCR™ Gold RNA-FISH/2ºIF enables straightforward multiplexing using 1-step quantitative signal amplification for all RNA and protein targets simultaneously.
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Any organism across the tree of life
Whether your RNA target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.​ For protein targets, use your own unmodified 1º antibodies of choice in combination with validated HCR™ 2º Antibody Probes (must use a different host species/isotype for each target protein).
Simultaneous Quantitative High-Resolution RNA and Protein Imaging
HCR™ Gold RNA-FISH/2ºIF enables RNA and protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.
1-step quantitative HCR™ Gold signal amplification for 10 target RNAs and proteins simultaneously
RNA and protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples
Getting started?
For multiplex quantitative HCR™ Gold RNA-FISH/2ºIF:
✓ order one HCR™ Gold RNA-FISH kit per RNA target
✓ use your own favorite 1º antibody for each target protein (different host species/isotype for each target protein)
✓ order one HCR™ Gold 2ºIF kit per target protein
✓ select a different HCR™ Gold Amplifier for each target RNA or protein