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HCR™ Pro RNA-ISH

Extreme-sensitivity RNA imaging with chromogenic or fluorescent staining

Enzymatic amplification for the most demanding targets/samples when signal-to-background is at an absolute premium (e.g., rapid scanning of a low-expression target in a highly-autofluorescent sample over a large field-of-view at low magnification)​.

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What comes in an HCR™ Pro RNA-ISH kit?​

  • HCR™ HiFi Probe backed by the HCR™ HiFi Probe Promise 

  • HCR™ HiFi Probe Hybridization Buffer

  • HCR™ HiFi Probe Wash Buffer

  • HCR™ Pro Amplifier kit​

    • Pretreat

    • HCR™ Pro Detect A​

    • HCR™ Pro Detect B​

    • HCR™ Pro Detect C​

    • HCR™ Pro Detect D​

    • HCR™ Pro Detect E​

    • HCR™ Pro Detect F HRP

    • Post-Process

  • HCR™ Pro Amplifier Wash Buffer

  • Matisse® chromogen kit (complimentary)

    • Matisse® Green or Matisse® Brown 

    • Matisse® Green Buffer or Matisse® Brown Buffer

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The HCR™ Pro RNA-ISH kit is used with an HRP chromogen for RNA-CISH (e.g., Matisse® Green or Matisse® Brown) or an HRP fluorogenic substrate for RNA-FISH (e.g., any of a variety of recommended third-party fluorescent tyramides).

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Safety Data Sheets

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HCR™ Pro RNA-CISH

Extreme-sensitivity chromogenic RNA imaging

The convenience of brightfield microscopy and the option of archival staining

Entirely protease-free workflow in tissue sections 
HCR™ Pro RNA-ISH leverages the unique sample penetration and triggered amplification properties of the HCR™ imaging platform to pioneer entirely protease-free workflows for chromogenic and fluorescent RNA in situ hybridization (RNA-CISH/RNA-FISH) in FFPE and fresh/fixed frozen tissue sections.

Native compatibility with IHC/IF
Traditional RNA imaging technologies rely heavily on protease pre-treatment to enable large imaging reagents to penetrate tissue sections, but this process often damages sample morphology and compromises protein target integrity, making it challenging to image RNA and protein targets in a single well-preserved sample. In contrast, the HCR™ Pro RNA-ISH protease-free workflow naturally preserves sample morphology and maintains protein target integrity, enabling seamless compatibility with existing immunohistochemistry (IHC)/immunofluorescence (IF) assays. 

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Infinite Catalog
Whether your target is already in our Infinite Catalog or just joining it, HCR™ HiFi Probes for any target RNA in any organism across the tree of life are designed by the MI Team using MI’s proprietary probe design process with no design fee.​
 

Getting started?

Request a complimentary HCR™ Pro RNA-ISH Starter Kit! Please register for an account and fill out this questionnaire. 

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