Multiplexed, quantitative, sensitive, versatile, robust. Learn more
✓ Simple
✓ Amplified
✓ Multiplexed
✓ Quantitative
✓ Penetrating
✓ Resolved
✓ Sensitive
✓ Portable
✓ Versatile
✓ Compatible
✓ Robust
2-stage enzyme-free protocol
Signal elevated above autofluorescence
Simultaneous 1-step amplification for up to 5 target RNAs
Signal scales linearly with target abundance
Whole-mount vertebrate embryos and thick tissue sections
Subcellular or single-molecule resolution as desired
Single molecules detected in thick autofluorescent samples
Suitable for use with diverse targets in diverse organisms
Multiple assay formats: RNA-FISH, RNA flow cytometry, northern blots
Combine with tissue clearing as desired
Automatic background suppression throughout the protocol
Quantitative RNA Analysis Modes with Automatic Background Suppression
HCR v3.0 probe sets, amplifiers, and buffers enable multiplexed quantitative RNA fluorescence in situ hybridization (RNA-FISH), RNA flow cytometry, and northern blotting with automatic background suppression throughout the protocol, dramatically enhancing performance and ease-of-use. Learn more
qHCR Imaging
Analog RNA relative quantitation with subcellular resolution in the anatomical context of thick autofluorescent samples. Learn more
dHCR Imaging
Digital RNA absolute quantitation with single-molecule resolution in the anatomical context of thick autofluorescent samples. Learn more
qHCR Flow Cytometry
Analog RNA relative quantitation for high-throughput expression profiling of mammalian and bacterial cells. Learn more
qHCR Northern Blots
Simultaneous quantification of RNA target size and abundance. Learn more
Any mRNA in Any Organism
Signal amplification based on the mechanism of hybridization chain reaction (HCR) leads to simple, robust, enzyme-free protocols offering the versatility to image any mRNA in any organism. Learn more
