HCR™ IF Protocols
Multiplexed, quantitative, high-resolution protein imaging
HCR™ probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution protein immunofluorescence (IF; how HCR™ IF works), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunostaining.
HCR™ IF: Protocol Overview
The same 2-stage protocol is used independent of the number of target proteins.
Simple Robust Protocols
HCR™ IF protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.
HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.
Plug-and-Play with Your 1º Antibodies of Choice
For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes.
Normal Sleep Schedule
We favor protocols with an overnight probe incubation step to enable researchers to maintain a normal sleep schedule; durations can be customized as desired based on sample thickness.
HCR™ IF + HCR™ RNA-FISH
HCR™ IF + HCR™ RNA-ISH protocols enable simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR™ signal amplification for all protein and RNA targets simultaneously.
Quantitative High-Resolution Protein Imaging
Subcellular protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.