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HCR™ IF + HCR™ RNA-FISH Technology

Simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging 

HCR™ IF probes, amplifiers, and buffers and HCR™ RNA-FISH probe sets, amplifiers, and buffers enable a unified approach to multiplexed, quantitative, high-resolution immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA-FISH).

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HCR™ IF + HCR™ RNA-FISH: How It Works

1. Protein Detection Stage

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2. RNA Detection Stage

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3. Amplification Stage

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1. Protein Detection Stage

Protein targets are detected with unlabeled primary antibody probes and initiator-labeled secondary antibody probes. 

2. RNA Detection Stage

RNA targets are detected with split-initiator DNA probes that colocalize full initiator i1 only when they bind specifically to the target. 

3. Amplification Stage

Initiators trigger self-assembly of tethered fluorescent amplification polymers for all protein and RNA targets simultaneously. 

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Quantitative 1-step enzyme-free signal amplification for all protein and RNA targets simultaneously

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Simple Robust Protocols

HCR™ IF + HCR™ RNA-FISH protocols are simple, robust, and enzyme-free, requiring only 3 stages independent of the number of protein and RNA targets.

Straightforward Multiplexing

HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all protein and RNA targets simultaneously.

Any organism across the tree of life

Free custom probe design for any target mRNA in any organism. For protein targets, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes.

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Simultaneous Quantitative High-Resolution Protein and RNA Imaging

Subcellular protein and RNA imaging enables protein and RNA relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

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The same 3-stage enzyme-free protocol is used independent of the number of protein and RNA targets

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Protein and RNA relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples

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Bidirectional quantitative protein and RNA discovery in an anatomical context

HCR™ IF + HCR™ RNA-FISH

HCR™ IF probes, amplifiers, buffers
HCR™ RNA-FISH probes, amplifiers, buffers
Multiplexed, quantitative, high-resolution
FFPE tissue sections
Whole-mount embryos

All major 

automation platforms 

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