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HCR™ IF + HCR™ RNA-FISH Products

Multiplexed, quantitative, high-resolution protein and RNA imaging

HCR™ probes, amplifiers, and buffers enable simultaneous multiplexed, quantitative, high-resolution immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA-FISH) (how HCR™ IF + HCR™ RNA-FISH works)

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Simple Robust Protocols

HCR™ IF + HCR™ RNA-FISH protocols are simple, robust, and enzyme-free, requiring only 3 stages independent of the number of protein and RNA targets.

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Straightforward Multiplexing

HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all protein and RNA targets simultaneously.

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Any organism across the tree of life

Free custom probe design for any target mRNA in any organism. For protein targets, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes.

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Simultaneous Quantitative High-Resolution Protein and RNA Imaging

Subcellular protein and RNA imaging enables protein and RNA relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

HCR™ IF Bundles

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Order one HCR™ IF bundle per protein target: initiator-labeled 2º antibody probe, amplifier, buffers. Safety Data Sheets

HCR™ RNA-FISH Bundles

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Order one HCR™ RNA-FISH bundle per RNA target: split-initiator DNA probe set, amplifier, buffers. Safety Data Sheets

Getting started?

For multiplexed quantitative HCR™ IF + HCR™ RNA-FISH: 

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 ✓ order one HCR™ IF bundle per protein target 

 ✓ order one HCR™ RNA-FISH bundle per RNA target

 ✓ use a different HCR™ amplifier and fluorophore for each target protein or RNA

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