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HCR™ IF + HCR™ RNA-FISH Products

Multiplexed, quantitative, high-resolution protein and RNA imaging

HCR™ probes, amplifiers, and buffers enable simultaneous multiplexed, quantitative, high-resolution immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA-FISH) (how HCR™ IF + HCR™ RNA-FISH works)

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Simple Robust Protocols

HCR™ IF + HCR™ RNA-FISH protocols are simple, robust, and enzyme-free, requiring only 3 stages independent of the number of protein and RNA targets.

Straightforward Multiplexing

HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all protein and RNA targets simultaneously.

Any organism across the tree of life

Free custom probe design for any target mRNA in any organism. For protein targets, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes.

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Simultaneous Quantitative High-Resolution Protein and RNA Imaging

Subcellular protein and RNA imaging enables protein and RNA relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

HCR™ IF Bundles

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Order one HCR™ IF bundle per protein target: initiator-labeled 2º antibody probe, amplifier, buffers. Safety Data Sheets

HCR™ RNA-FISH Bundles

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Order one HCR™ RNA-FISH bundle per RNA target: split-initiator DNA probe set, amplifier, buffers. Safety Data Sheets

Getting started?

For multiplexed quantitative HCR™ IF + HCR™ RNA-FISH: 

 ✓ order one HCR™ IF bundle per protein target 

 order one HCR™ RNA-FISH bundle per RNA target

 ✓ use a different HCR™ amplifier and fluorophore for each target protein or RNA

All major 

automation platforms 

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