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HCR™ IF Products

Multiplexed, quantitative, high-resolution protein imaging

HCR™ probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunofluorescence (IF; how HCR™ IF works), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunostaining.

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HCR™ Probe

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Initiator-labeled 2º antibody probes are quality-control tested by the Molecular Instruments Team.

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Plug-and-play using your own 1º antibody of choice without modification.

HCR™ Amplifier

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Metastable fluorescent hairpins are quality-control tested by the Molecular Instruments Team. 

HCR™ Buffers

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Antibody buffer

Amplification buffer

HCR™ IF Bundle

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Conveniently order reagents as a bundle containing any of: probe, amplifier, buffers. Safety Data Sheets

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Simple Robust Protocols

HCR™ IF protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.

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Straightforward Multiplexing

HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.

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Plug-and-Play with Your 1º Antibodies of Choice

For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes. 

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HCR™ Protein-IF + HCR™ RNA-FISH

Combine HCR™ protein-IF bundles with HCR™ RNA-FISH bundles for simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR™ signal amplification for all protein and RNA targets simultaneously. 

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Quantitative High-Resolution Protein Imaging

Subcellular protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

Getting started?

For multiplexed, quantitative, high-resolution HCR™ IF:

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 ✓ use your own 1º antibody of choice for each target protein (each 1º antibody raised in a different host organism) 

 ✓ order one HCR™ 2º antibody probe per target (each 2º antibody probe for use with a different 1º antibody and HCR™ amplifier)

 ✓ order one HCR™ amplifier per target (each with a different fluorophore)

 ✓ order HCR™ buffers

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