HCR RNA-FISH: Protocol Overview
The same 2-stage protocol is used independent of the number of target RNAs.
HCR™ IF Products
Multiplexed, quantitative, high-resolution protein imaging
HCR™ probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunofluorescence (IF; how HCR™ IF works), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunostaining.
HCR™ Probe
Initiator-labeled 2º antibody probes are quality-control tested by the Molecular Instruments Team.
​
Plug-and-play using your own 1º antibody of choice without modification.
HCR™ Amplifier
Metastable fluorescent hairpins are quality-control tested by the Molecular Instruments Team.
HCR™ Buffers
Antibody buffer
Amplification buffer
HCR™ IF Bundle
Conveniently order reagents as a bundle containing any of: probe, amplifier, buffers. Safety Data Sheets
Simple Robust Protocols
HCR™ IF protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.
​
Straightforward Multiplexing
HCR™ enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.
​
Plug-and-Play with Your 1º Antibodies of Choice
For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR™ 2º antibody probes.
​
HCR™ Protein-IF + HCR™ RNA-FISH
Combine HCR™ protein-IF bundles with HCR™ RNA-FISH bundles for simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR™ signal amplification for all protein and RNA targets simultaneously.
Quantitative High-Resolution Protein Imaging
Subcellular protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.
Getting started?
For multiplexed, quantitative, high-resolution HCR™ IF:
​
✓ use your own 1º antibody of choice for each target protein (each 1º antibody raised in a different host organism)
✓ order one HCR™ 2º antibody probe per target (each 2º antibody probe for use with a different 1º antibody and HCR™ amplifier)
✓ order one HCR™ amplifier per target (each with a different fluorophore)
✓ order HCR™ buffers
All major
automation platforms
