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HCR™ Gold User Guides

Each HCR™ Gold User Guide contains kit information, a workflow overview, validated protocols for relevant sample types, common recipes, FAQ, and technology citation notes:

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HCR™ Gold IF

Game-Changing Multiplex Immunofluorescence

Use Your Trusted 1º Antibodies for Same-Species Multiplexing with the Breakthrough HCR™ HiFi Encoder 

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A new era for in situ hybridization:

10-plex, quantitative, high-resolution, sensitive, versatile, robust, enzyme-free

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RNA relative quantitation for high-throughput expression profiling of mammalian cells and bacteria

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Combine HCR™ Gold RNA-FISH and HCR™ Gold 2ºIF (using different-species 1º antibodies for each target protein) 

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Game-changing multiplex immunofluorescence using same-species 1º antibodies with the HCR™ HiFi Encoder

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Simultaneous quantification of RNA target size and abundance

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Quantitative 1-step enzyme-free HCR™ Gold signal amplification for 10 RNA and/or protein targets simultaneously

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Multiplex, quantitative, high-resolution IF using different-species 1º antibodies with HCR™ 2º Antibody Probes  

Getting started?

Request a complimentary HCR™ Gold RNA-FISH Starter Kit! Please register for an account and fill out this questionnaire. 

For game-changing multiplex protein imaging using HCR™ Gold IF:​

 âœ“ use your own favorite 1º antibodies (same host species/isotype for each target protein if desired)

 âœ“ order one HCR™ Gold IF kit per target protein (select a different HCR™ HiFi Encoder and HCR™ Gold Amplifier for each target protein)

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