HCR™ Gold 2ºIF
Multiplex, quantitative, high-resolution protein imaging
Use your favorite 1º antibodies without modification (must use a different host species/isotype for each target protein) in combination with validated HCR™ 2º Antibody Probes
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What comes in an HCR™ Gold 2ºIF kit?
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HCR™ 2º Antibody Probe
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HCR™ Antibody Buffer
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HCR™ Gold Amplifier
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HCR™ Gold Amplifier Buffer
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HCR™ Gold Amplifier Wash Buffer
Simple Robust Protocols
HCR™ Gold 2ºIF protocols are simple, robust, and enzyme-free, requiring only 2 stages (probe binding, amplification) independent of the number of target proteins
Straightforward Multiplexing
HCR™ Gold 2ºIF enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.
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Use Your 1º Antibodies of Choice
For protein targets in any organism across the tree of life, use your own unmodified 1º antibodies of choice in combination with validated HCR™ 2º Antibody Probes (must use a different host species/isotype for each target). ​
Quantitative High-Resolution Protein Imaging
HCR™ Gold 2ºIF enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.
1-step quantitative HCR™ Gold signal amplification for all target proteins simultaneously
Protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples
Getting started?
For multiplex, quantitative, high-resolution HCR™ Gold 2ºIF:
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✓ use your own favorite 1º antibodies (different host species/isotype for each target protein)
✓ order one HCR™ Gold 2ºIF kit per target protein (select a different HCR™ 2º Antibody Probe and HCR™ Gold Amplifier for each target protein)