HCR™ Gold 2ºIF
Multiplex, quantitative, high-resolution protein imaging
Use your favorite 1º antibodies without modification (must use a different host species/isotype for each target protein) in combination with validated HCR™ 2º Antibody Probes
What comes in an HCR™ Gold 2ºIF kit?
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HCR™ 2º Antibody Probe
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HCR™ Antibody Buffer
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HCR™ Gold Amplifier
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HCR™ Gold Amplifier Buffer
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HCR™ Gold Amplifier Wash Buffer
Simple Robust Protocols
HCR™ Gold 2ºIF protocols are simple, robust, and enzyme-free, requiring only 2 stages (probe binding, amplification) independent of the number of target proteins
Straightforward Multiplexing
HCR™ Gold 2ºIF enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.
Use Your 1º Antibodies of Choice
For protein targets in any organism across the tree of life, use your own unmodified 1º antibodies of choice in combination with validated HCR™ 2º Antibody Probes (must use a different host species/isotype for each target).
Quantitative High-Resolution Protein Imaging
HCR™ Gold 2ºIF enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.
1-step quantitative HCR™ Gold signal amplification for all target proteins simultaneously
Protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples
Getting started?
For multiplex, quantitative, high-resolution HCR™ Gold 2ºIF:
✓ use your own favorite 1º antibodies (different host species/isotype for each target protein)
✓ order one HCR™ Gold 2ºIF kit per target protein (select a different HCR™ 2º Antibody Probe and HCR™ Gold Amplifier for each target protein)