HCR IHC Protocols

Multiplexed, quantitative, high-resolution protein imaging

HCR probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution protein immunohistochemistry (IHC; how HCR IHC works), immunofluorescence (IF), immunocytochemistry (ICC), and immunostaining.

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HCR IHC: Protocol Overview

Detection Stage

Amplification Stage

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The same 2-stage protocol is used independent of the number of target proteins.

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Experience Multiplexed, Quantitative, High-Resolution Immunohistochemistry

Amplified. Enzyme-Free. FFPE. Simple.

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Simple Robust Protocols

HCR IHC protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.

Straightforward Multiplexing

HCR enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.

Plug-and-Play with Your 1º Antibodies of Choice

For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR 2º antibody probes. 

Normal Sleep Schedule

We favor protocols with an overnight probe incubation step to enable researchers to maintain a normal sleep schedule; durations can be customized as desired based on sample thickness.

HCR IHC + HCR RNA-FISH

HCR IHC + HCR RNA-ISH protocols enable simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR signal amplification for all protein and RNA targets simultaneously. 

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Quantitative High-Resolution Protein Imaging

qHCR protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

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