HCR RNA-FISH: Protocol Overview
The same 2-stage protocol is used independent of the number of target RNAs.
HCR IHC Products
Multiplexed, quantitative, high-resolution protein imaging
HCR probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunohistochemistry (IHC; how HCR IHC works), immunofluorescence (IF), immunocytochemistry (ICC), and immunostaining.
HCR Probe
Initiator-labeled 2º antibody probes are quality-control tested by the Molecular Instruments Team.
Plug-and-play using your own 1º antibody of choice without modification.
HCR Amplifier
Metastable fluorescent HCR hairpins are quality-control tested by the Molecular Instruments Team.
HCR Buffers
Antibody buffer
Amplification buffer
HCR IHC Bundle
Conveniently order reagents as a bundle containing any of: probe, amplifier, buffers. Safety Data Sheets
Simple Robust Protocols
HCR IHC protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.
Straightforward Multiplexing
HCR enables straightforward multiplexing using 1-step quantitative signal amplification for all target RNAs simultaneously.
Plug-and-Play with Your 1º Antibodies of Choice
For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR 2º antibody probes.
HCR Protein-IHC + HCR RNA-FISH
Combine HCR protein-IHC bundles with HCR RNA-FISH bundles for simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR signal amplification for all protein and RNA targets simultaneously.
Quantitative High-Resolution Protein Imaging
qHCR protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.
Getting started?
For multiplexed, quantitative, high-resolution HCR IHC:
✓ use your own 1º antibody of choice for each target protein (each 1º antibody raised in a different host organism)
✓ order one HCR 2º antibody probe per target (each 2º antibody probe for use with a different 1º antibody and HCR amplifier)
✓ order one HCR amplifier per target (each with a different fluorophore)
✓ order HCR buffers