HCR IHC Products

Multiplexed, quantitative, high-resolution protein imaging

HCR probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunohistochemistry (IHC; how HCR IHC works), immunofluorescence (IF), immunocytochemistry (ICC), and immunostaining.

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HCR Probe

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Initiator-labeled 2º antibody probes are quality-control tested by the Molecular Instruments Team.

Plug-and-play using your own 1º antibody of choice without modification.

HCR Amplifier

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Metastable fluorescent HCR hairpins are quality-control tested by the Molecular Instruments Team. 

HCR Buffers

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Antibody buffer

Amplification buffer

HCR IHC Bundle

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Conveniently order reagents as a bundle containing any of: probe, amplifier, buffers. Safety Data Sheets

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Simple Robust Protocols

HCR IHC protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.

Straightforward Multiplexing

HCR enables straightforward multiplexing using 1-step quantitative signal amplification for all target RNAs simultaneously.

Plug-and-Play with Your 1º Antibodies of Choice

For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR 2º antibody probes. 

HCR Protein-IHC + HCR RNA-FISH

Combine HCR protein-IHC bundles with HCR RNA-FISH bundles for simultaneous multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR signal amplification for all protein and RNA targets simultaneously. 

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Quantitative High-Resolution Protein Imaging

qHCR protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

Getting started?

For multiplexed, quantitative, high-resolution HCR IHC:

 ✓ use your own 1º antibody of choice for each target protein (each 1º antibody raised in a different host organism) 

 ✓ order one HCR 2º antibody probe per target (each 2º antibody probe for use with a different 1º antibody and HCR amplifier)

 order one HCR amplifier per target (each with a different fluorophore)

 ✓ order HCR buffers