HCR IHC Technology
Multiplexed, quantitative, high-resolution protein imaging
HCR probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunohistochemistry (IHC), immunofluorescence (IF), immunocytochemistry (ICC), and immunostaining.
HCR IHC: How It Works
Protein targets are detected with unlabeled primary antibody probes and HCR initiator-labeled secondary antibody probes.
An HCR amplifier comprises metastable fluorescent HCR hairpins h1 and h2. HCR initiator i1 triggers self-assembly of tethered fluorescent HCR amplification polymers.
Simple Robust Protocols
HCR IHC protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.
HCR enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.
Plug-and-Play with Your 1º Antibodies of Choice
For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR 2º antibody probes.
HCR Protein-IHC + HCR RNA-FISH
Combine HCR IHC probes with HCR RNA-FISH probes to enable HCR protein-IHC + HCR RNA-FISH for multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR signal amplification for all protein and RNA targets simultaneously.
Quantitative High-Resolution Protein Imaging
qHCR protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.