HCR IHC Technology

Multiplexed, quantitative, high-resolution protein imaging

HCR probes, amplifiers, and buffers enable multiplexed, quantitative, high-resolution immunohistochemistry (IHC), immunofluorescence (IF), immunocytochemistry (ICC), and immunostaining.

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HCR IHC: How It Works

Detection Stage

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Amplification Stage

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Detection Stage

Protein targets are detected with unlabeled primary antibody probes and HCR initiator-labeled secondary antibody probes. 

Amplification Stage

An HCR amplifier comprises metastable fluorescent HCR hairpins h1 and h2. HCR initiator i1 triggers self-assembly of tethered fluorescent HCR amplification polymers. 

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Experience Multiplexed, Quantitative, High-Resolution
Immunohistochemistry

Amplified. Enzyme-Free. FFPE. Simple.

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Simple Robust Protocols

HCR IHC protocols are simple, robust, and enzyme-free, requiring only 2 stages independent of the number of target proteins.

Straightforward Multiplexing

HCR enables straightforward multiplexing using 1-step quantitative signal amplification for all target proteins simultaneously.

Plug-and-Play with Your 1º Antibodies of Choice

For protein targets in any organism across the tree of life, plug-and-play using your own 1º antibodies of choice (without modification) in combination with validated HCR 2º antibody probes. 

HCR Protein-IHC + HCR RNA-FISH

Combine HCR IHC probes with HCR RNA-FISH probes to enable HCR protein-IHC + HCR RNA-FISH for multiplexed, quantitative, high-resolution protein and RNA imaging with 1-step HCR signal amplification for all protein and RNA targets simultaneously. 

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Quantitative High-Resolution Protein Imaging

qHCR protein imaging enables protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples.

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The same 2-stage enzyme-free protocol is used independent of the number of target proteins

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Protein relative quantitation with subcellular resolution in the anatomical context of highly autofluorescent samples

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Bidirectional quantitative protein discovery in an anatomical context

HCR IHC

HCR initiator-labeled 2º antibody probes, HCR amplifiers, HCR buffers
Plug-and-play with your own 1º antibodies of choice (without modification) 
Multiplexed, quantitative, high-resolution
FFPE tissue sections
Whole-mount embryos